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The European Food Safety Authority recommends healthy adults limit caffeine to 400 mg daily (approximately four cups of coffee)

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Biochemical Parameters For biochemical analysis, the blood samples collected at the end of the experiment were used to measure glucose, triacylglycerol, total cholesterol, and high-density lipoprotein-cholesterol (HDL), alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and albumin levels all by Labtest Kits (Lagoa Santa, MG, Brazil)