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The knock-in efficiency was determined using the equation: Knock-in (%) = (Copy number of target)/(Copy number of reference + Copy number of target) 100

While GLP-1s represent a major clinical advancement, the broad demand for a high-priced medicine creates a level of financial strain that is difficult to absorb at scale

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J Nutr 150(7):18451851 [DOI] [PMC free article] [PubMed] [Google Scholar] 46.Farsijani S, Morais JA, Payette H, Gaudreau P, Shatenstein B, Gray-Donald K et al (2016) Relation between mealtime distribution of protein intake and lean mass loss in free-living older adults of the NuAge study

As there is no meaningful expression of GLP-1 receptors on hepatocytes, the major effect of GLP-1 agonists on MASH is likely to be indirect and may be related to a reduction in calorie intake, body weight and insulin resistance, all of which would lead to reduced liver lipid accumulation and hepatic inflammation 7,8

Insulin is physiologically constantly secreted to a small extent during the fasting state in order to enhance glucose uptake by the peripheral tissues