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The most important factor remains consistent adherence to the prescribed schedule, regardless of the specific time chosen

Avoiding carbohydrates for 60 to 90 minutes before and after GH secretagogue administration prevents the insulin spike that suppresses growth hormone release

Whole blood samples were collected from the tail vein in 20-l heparinized capillary tubes (Fisher Scientific company LLC #22-362566), anesthesia was induced using isoflurane, the blood samples were transferred quickly (10 s) to 2-ml gas chromatographic septum sealed vials (Sigma #29381-U) containing 200 l of an internal standard solution consisting of 0.6 N perchloric acid and 4 mM n-propanol in double distilled water on ice