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Cells were imaged 24 hours after seeding in RPMI-1640 buffer without phenol red 100 nM exendin-4 by time-lapse microscopy every 12 seconds for 11 minutes in a 37 C heated chamber using a Leica Stellaris 8 inverted confocal microscope with a 40/1.2 NA oil immersion objective with excitation wavelengths of 440 and 580 nm and emission wavelength of 620 nm

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