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Shaking creates violent agitation that introduces air bubbles throughout the solution and generates shear forces that can denature the peptide

Alfradi Mulihano, Clare OBrien, MD

USPSTF, United States Preventive Service Task Force

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J Hepatol 77(3):607618 Andrews TS, Atif J, Liu JC, Perciani CT, Ma XZ, Thoeni C, Slyper M, Eraslan G, Segerstolpe A, Manuel J, Chung S, Winter E, Cirlan I, Khuu N, Fischer S, Rozenblatt-Rosen O, Regev A, McGilvray ID, Bader GD, MacParland SA (2022) Single-cell, single-nucleus, and spatial RNA sequencing of the human liver identifies cholangiocyte and mesenchymal heterogeneity