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Methods Cell culture, expression constructs, and transfection MIN-6 (purchased from ATCC) cells and GLUTag cells (obtained from Takashi Tsuboi, the university of Tokyo, Japan) were cultured in DMEM with 10% (v/v) heat-inactivated fetal bovine serum

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GLP-1 receptor stimulation may prevent the increase in ROS induced by ANG II

Furthermore, a double Ala-scan of ECL2 also identified a number of sites likely to be important in forming the binding site of the peptide's N-terminus (Table 1) and are also likely to be important for the agonist-induced activation of the receptor (Mann et al ., 2010a,b)