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To obtain pressure measurements, a balloon-tipped catheter (Swann-Ganz catheter, PA catheter) is inserted through a vein and advanced through the heart and wedged in the pulmonary artery to estimate left atrial pressure

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After sample loading, the column was washed with 10 mL 15% ACN + 0.1% TFA, and the core peptide was eluted with 8 mL 60% ACN + 0.1% TFA and lyophilized