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[1] The development of these two classes of drugs was based upon observations from the early twentieth century when it was noted that factors secreted from the gut participated in the regulation of pancreatic endocrine secretion

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The HEK293 cell line expressing a functional mouse GLP-1R and the HEK293 cell line (generated in-house using HEK293 cell line purchased from ATCC) expressing a functional human glucagon receptor were cultured in DMEM supplemented with 10% fetal bovine serum and 10 g ml 1 blasticidin

doi: 10.1016/j.physbeh.2014.03.011 129 LeeSJKriegerJPVergaraMQuinnD

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While electrolytes will not eliminate all of these, adequate mineral status reduces their severity in most cases