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The eluted sample was quickly neutralized by addition of 1/10 volume of 1 M Tris pH 8.0 and further polished on a size-exclusion chromatography column (Superdex 75, GE healthcare) pre-equilibrated with phosphate-buffered saline (PBS, pH 7.4)

de Heide, L

9.4% with placebo

Clinical trials show that diarrhea occurs in approximately 19-30% of patients using these medications for weight management

Dietitians noted that patients often do not understand how these therapies work

The CPRD contains data on prescriptions written by general practitioners.21 The HES APC database archives hospital admission records of patients treated within English National Health Service hospitals.2223 The ONS Death Registration database is a vital statistics database used as the gold standard to identify patients who died from suicide and other causes during follow-up.222425 Diagnoses in the HES APC and ONS are recorded using the ICD-10 (international classification of diseases, 10th revision) classification