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GLP-1 projections from NTS to NAc and VTA promote satiation and reduce food reward, while orexinergic projection from lateral hypothalamus to NTS suppresses satiation and increases food reward (Dossat et al., 2011)

doi:10.1056/nejmoa2208601

Clonal HEK293 cells stably expressing either untagged human GIPR or human GLP-1R at various levels of receptor density were used for measurement of cAMP accumulation using the Gs Dynamic Assay and homogenous time-resolved fluorescence (62AM4PEJ, PerkinElmer)

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Severe anaphylactic reactions with GLP-1 receptor agonists have not been reported in Pubmed