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Central administration of GLP-1-(736) amide inhibits food and water intake in rats

Dry mouth during semaglutide treatment may result from indirect factors such as reduced fluid intake due to appetite suppression, dehydration from gastrointestinal side effects, or concurrent medications that cause xerostomia rather than a direct effect of semaglutide itself

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All peptides and peptide drug conjugates were desalted by three consecutive cycles of redissolving the peptide in 0.001 M aqueous HCl followed by lyophilization

Immunohistochemical detection of alpha and beta cells was performed using rabbit anti-insulin (3014, 1:800, Cell Signaling Technology) and mouse anti glucagon (G2654, 1:1,000, Merck) as primary antibodies and goat anti-rabbit AF750 (A21039, 1:100, Invitrogen) and donkey anti-mouse AF555 (A32773, 1:200, Invitrogen) as secondary antibodies