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Materials and methods HepG2 culture We obtained the human hepatoma HepG2 cell line (HB-8065, ATCC) from ATCC (Manassa, Virginia, USA) and maintained it in Dulbecco's modified Eagle's medium (DMEM) (31966047, Gibco, Massachusetts, USA) at 37 C and 5% CO2

All patients were diagnosed with AD before the trial

Injection site rotation strategies for sustained weight loss Rotation is not optional
doi: 10.1152/ajpendo.00137.2013
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