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Materials and methods HepG2 culture We obtained the human hepatoma HepG2 cell line (HB-8065, ATCC) from ATCC (Manassa, Virginia, USA) and maintained it in Dulbecco's modified Eagle's medium (DMEM) (31966047, Gibco, Massachusetts, USA) at 37 C and 5% CO2

All interventions were compared with current use of SGLT2is (20% of the total population) and GLP-1 RAs (5% of the total population)

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Short acting GLP-1 receptor agonists may cause a brief increase in heart rate, while some long-acting GLP-1 receptor agonists may cause a sustained increase in circadian heart rate