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The NNT for tirzepatide was 1.23 for achieving 10% weight loss, 1.55 for 15%, 2.07 for 20%, 3.16 for 25%, and 5.08 for 30%

In support of this, ICV administration of GLP-1 in rats has been shown to attenuate the fasting-induced rise in NPY/AgRP expression and decreases fasting-induced inhibition of POMC/CART expression in the ARC (Seo et al., 2008)

REFERENCES The increase in appetite for obesity drugs

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Step 2: Add 100ul standard/sample/biotin-labeled antigen working solution into each well

Up to 40% of total weight lost on GLP-1 drugs is lean mass (muscle), not fat