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doi: 10.3390/jcm13164674 30 YunJ-SKoS-H

Formalin-fixed paraffin-embedded brain sections (3 sections per mouse per triplex) underwent RNAscope in situ RNA hybridization (ISH) for the detection of Lepr (ACDBio probe #418858-C2), Glp-1r (ACDBio probe #418858-C2), Gipr (ACDBio probe #319128-C1), Pomc (ACDBio probe #314088-C2) and Agrp (ACDBio probe #400718-C1), followed by immunofluorescence (IF) staining for pSTAT3 protein (Cell Signaling anti-pSTAT3 #9145)

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Furthermore, through the activation of canonical G-protein coupled receptors, GLP-1 receptors activate adenylyl cyclase which leads to an increased production of cAMP, a second messenger involved in vasodilation [27]

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Fructose induces glucose-dependent insulinotropic polypeptide, glucagon-like peptide-1 and insulin secretion: Role of adenosine triphosphate-sensitive K(+) channels