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Formalin-fixed paraffin-embedded brain sections (3 sections per mouse per triplex) underwent RNAscope in situ RNA hybridization (ISH) for the detection of Lepr (ACDBio probe #418858-C2), Glp-1r (ACDBio probe #418858-C2), Gipr (ACDBio probe #319128-C1), Pomc (ACDBio probe #314088-C2) and Agrp (ACDBio probe #400718-C1), followed by immunofluorescence (IF) staining for pSTAT3 protein (Cell Signaling anti-pSTAT3 #9145)
The C-terminal of GLP-1 binds to the extracellular domain, whereas the N-terminal binds to the transmembrane domain of the GLP-1 receptors
However, in spite of a lower limit of sensitivity of Caco-2 monolayers to drug permeability enhancement, in the literature, Caco-2 cells have been highly sensitive to excipient effects on drug permeability compared to in vivo (54)
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Service GJ, Thompson GB, Service FJ, Andrews JC, Collazo-Clavell ML, Lloyd RV
