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First, the cells or tissues are disrupted by grinding or homogenization in buffered isotonic solutions, commonly at physiologic pH and at 4C to minimize protein denaturation during purification

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However, they are also more susceptible to rapid elimination and may lead to higher reactivity and toxicity due to their larger surface area

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10.2164/jandrol.05114 153 TurriF.CapraE.LazzariB.CremonesiP.StellaA.PizziF

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