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Ribonucleic acid isolation and real-time polymerase chain reaction Total ribonucleic acid (RNA) was extracted from each of three samples, cultured cells, snap-frozen pancreas, and enucleated pancreatic tumors of Pdx1-Cre;Trp53 R172H ;Rb f/f mice (n = 10) using an RNA isolation kit (Qiagen, Valencia, CA, USA) 38 , following the manufacturers instructions

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