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Protective effects of NAMPT or MAPK inhibitors and NaR on Wallerian degeneration of mammalian axons

The clarified cell lysate was either directly submitted to further protein purification steps or flash-frozen in liquid nitrogen after addition of 10% glycerol and stored at 80 C for use in biocatalytic reactions

PMID: 33667417

Advani SM, Lee MS, Overman MJ, Fogelman D, Kee BK, Manuel SD, Davis J, Morris VK, Clarke CN, Daniel CR, Menter DG, Hamilton SR, Maru DM, Kopetz S

Hypoxic conditions within tumors stimulate angiogenesis by upregulating hypoxia-inducible factor-1 (HIF-1), which in turn induces the production of vascular endothelial growth factor (VEGF)

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