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sgRNAs against exons 4 and 5 of the Gipr , Glp-1r and Gcgr genes (ESM Table 2) were designed using CRISPOR tool (www.crispor.tefor.net), in vitro transcribed (E3322, NEB) and purified using the MEGAclear Kit
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Now, however, those compounded versions will no longer be legal
So the path looks indirect
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