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Couplings were performed with Fmoc-amino acid (2.0 mL, 0.5 M in DMF), DIC (1.0 mL, 2.0 M in DMF) and Oxyma (1.75 mL, 0.50 M in DMF) for 30 seconds at room temperature followed by 4 min at 90 C

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This measured approach often reduces the frequency and severity of adjustment-related headaches compared to rapid-escalation protocols

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