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Consistent with the restoration of lipid catabolic gene expression, GSEA demonstrated significant enrichment of fatty acid metabolism (NES = 1.575, P = 0.0061) and fatty acid degradation pathways (NES = 1.729, P = 0.0020) in the HFD + Ret group compared with the HFD group

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When given to DIO mice, both peptides led to significant weight reduction, improved glucose tolerance, increased energy expenditure, and reductions in plasma cholesterol and liver steatosis

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FIGURE 10 3.6 Validation of the expression of GLP-1 signaling-related genes in CRC tissues Subsequently, we performed protein-protein interaction network analysis among 42 genes related to GLP-1 signaling (Supplementary Figure S1) and selected top 10 genes with highest node-degree for further validation in CRC tissues (Supplementary Table S2)

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