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( I ) cAMP levels in cell lysates were determined following pretreatment with 100 nM each of GLP-1(2836), scrambled control, or forskolin in siRNA-treated cells (white bars) versus the scrambled siRNA control (gray bars) ( n = 3/group for all cAMP and LDH assays, each in triplicate)

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Recent investigations from 2023 to 2025 highlight several key findings: microneedle-based delivery consistently reduced neuropathic pain [94], improved nerve function through precise local drug application [95], promoted regeneration when combined with stem cellderived products or electrically responsive systems [96], and decreased inflammation when loaded with anti-inflammatory agents [97]

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Here, we identified that DPP4 in hepatocytes but not TEK receptor tyrosine kinasepositive endothelial cells regulates the local bioactivity of incretin hormones and gluconeogenesis