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Dapagliflozin-induced weight loss affects 24-week glycated haemoglobin and blood pressure levels

(a) Graphical representation of food responding procedure, showing mouse in operant chamber permitted to respond for food pellets (20 mg)

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Understanding these mechanisms helps distinguish between expected physiological changes and symptoms requiring clinical assessment

Equal amounts of protein (30 g) were loaded onto 10% SDS-PAGE gel, then electrophoresed and transferred to 0.2 m PVDF membrane (Millipore, Darmstadt, Germany), which were blocked with 5% BSA for 2 h and incubated with the following primary antibodies overnight at 4C: against COX-2 (1:1000), iNOS (1:2000), p-NF-B p65 (1:1000), NF-B P65 (1:1000), p-AKT (1:2000), AKT (1:2000), p-P38 (1:2000), P38 (1:1000), p-ERK1/2 (1:2000), ERK1/2 (1:2000), p-JNK (1:2000), JNK (1:2000), and -actin (1:2000)

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