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Similarly, secretin, although less studied for weight loss, regulates digestion, fat absorption and digestion, that may provide broader benefits for metabolic therapies

The eluted sample was quickly neutralized by addition of 1/10 volume of 1 M Tris pH 8.0 and further polished on a size-exclusion chromatography column (Superdex 75, GE healthcare) pre-equilibrated with phosphate-buffered saline (PBS, pH 7.4)

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This step aimed to reduce the masking effect of gastrointestinal events to avoid possible competitive bias

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It's important to note that the difference in these results may be due in part to variations in dosage