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The Lancet , 398 (10314), 1807-1817

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Afterward, the cells were washed with internalization buffer (HBBS supplemented with 1 mM CaCl 2 , 2.5 mM MgCl 2 , 20 mM HEPES, and 0.1% Pluronic F-68, pH 7.4) followed by addition of 100 M preheated fluorescein-O-acetic acid (acceptor, Sigma-Aldrich)

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The bottom line: treating dosing accuracy as a cost control measure reframes the entire preparation process

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