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All mice were euthanized at 56 days after treatment, tracheal cannulated and lavaged three times with 0.5 ml of sterile cold phosphate-bufered saline (PBS) to collect the bronchoalveolar lavage fluid (BALF)
Briefly, PathHunter EA--Arrestin CHO cells expressing the GLP-1R (DiscoveRx) were seeded overnight in white 384-well plates at 10,000 cells per well in 20 l of F12 medium and incubated overnight

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