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[DOI] [PMC free article] [PubMed] [Google Scholar] 130.Brindisi M.C., Brondel L., Meillon S., Barthet S., Grall S., Fenech C., Linard F., Schlich P., Astruc K., Mouillot T., et al

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Materials and methods Solutions, drugs, and chemicals Smooth muscle buffer (SMB) was prepared in house and contained 120 mM NaCl, 4 mM KCl, 2.6 mM KH 2 PO 4 , 2 mM CaCl 2 , 25 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethanesulfonic acid), 14 mM glucose, and 2.1% essential amino acid mixture (pH was adjusted to 7.4)