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The Mytho gene was also subcloned in the pEGFP-N3 vector (4.7 kb) (Addgene) with KOD Hot Start DNA polymerase using the following primers with sticky ends: Fw 5-AAAGCTAGCATGCTGGACCTGGAGGTGGT-3 and Rw compl

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This introduces potential variability in patient populations and study designs, suggesting a need for future trials directly comparing these treatments

Interestingly, human phosphatase and tensin homolog (PTEN) mutations that lead to decreased PTEN expression increase the risk of obesity (Pal et al

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