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Protein bands were visualized using WesternBright Quantum (Advansta, Menlo Park, California, USA) and quantified by a ChemiDoc XRS + system with Image Lab software (Bio-Rad, Hercules, CA, USA)

Standard escalation protocol The most widely used escalation schedule for compounded tirzepatide B12 formulations follows this structure

Patients should report ongoing gastrointestinal symptoms, signs of dehydration, or thirst that seems disproportionate to fluid intake

GLP-1 agonists mimic the GLP-1 hormone, helping regulate insulin, glucose, and appetite

Other patients with absorption deficiencies like celiac or those with history of gastric bypass surgery should also discuss with their doctor if treatment with a GLP-1 is right for them

SB: Data curation, Writing original draft, Writing review & editing, Conceptualization