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Weeks 5 to 8: Increasing to 5 mg After completing four weeks at 2.5 mg, youll double to 5 mg weekly

cAMP accumulation assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were diluted to the density of 6 10 5 cells/ml and INS-1(832/13) cells to 2 10 6 cells/ml in stimulation buffer [phenol-free DMEM containing 5 mM HEPES, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) and 0.1% (w/v) BSA], and cAMP detection was carried out as described previously 48

Track any foods that seem to trigger episodes

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And that rhythm