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In brief, 5-m sections were baked at 60 C for 32 minutes and then deparaffinized at 72 C for 24 minutes

This N-terminal insertion drives conformational changes in the TMD, including the outward movement of transmembrane helix 6 (TM6) and reorganization of TM5 and TM7, that create a binding surface for heterotrimeric G proteins on the intracellular face of the receptor

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