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Subsequently, 200 l of chloroform was added per 1 ml of TRI reagent (MRC, TR118), the tube was vortexed for 15 s, and the mixture was incubated at room temperature for 23 min

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Both studies examined the effect of GLP-1RA on fibroblast-like synoviocytes (FLSs), cells in the synovial fluid of joints that normally play a protective and homeostatic role in joint function but can transform into a pathological phenotype that is apoptosis-resistant and proinflammatory in function in the presence of inflammatory stimuli

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Since these receptors are present in various organ systems and in the brain, their roles in satiety and appetite regulation, as well as neuroinflammation and neuroplasticity, must be considered