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Co-immunoprecipitation INS-1 832/3 cells previously transfected with the relevant plasmids, INS-1 832/3 SNAP/FLAG-hGLP-1R or SNAP/FLAG-hGIPR cells were seeded in 6-well plates at 1.2 10 6 cells per well and incubated overnight

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Compared to continued use, they found that stopping or interrupting GLP-1 treatment for as little as six months was linked to a significant increase in the risk of major cardiovascular events

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This has direct implications for semaglutide users concerned about skin elasticity during weight loss