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Metformin-Induced Increases in GDF15 Are Important for Suppressing Appetite and Promoting Weight Loss

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The whole brain and cervical spinal cord (C1-C2) were collected and postfixed with 4 % PFA overnight at 4 C.The medullary segment including the TNC between + 1 and 3 mm from the obex was removed and transferred to 30 % sucrose for 48 h