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Furthermore, laboratory and physiological measurements, including body weight, hemoglobin A1c, and blood pressure, were only available for subsets of patients, which may have affected some analyses

Briefly, after blocking with 10% goat serum, 20 m cryosections were incubated with goat anti-mouse IgG conjugated with Alexa488 (1:50, Life Technologies) for 20 h at 4 C

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The lowest dose is considered an introductory dose designed to prepare the body for the medications effects and mitigate adverse events, which are usually dose-dependent and more prominent at higher doses

This structural basis underlies the ligand-specific signaling bias, without being an eitheror selectivity

But a real-world application for peptides derived from Gila monster venom wasnt even a consideration when Raufman began his toxicity tests