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Materials and methods GLP-1 peptides GLP-1 peptides, semaglutide (HY-114118, MedChemExpress, Monmouth Junction, NJ, USA), tirzepatide (39748, Cayman Chemical, Ann Arbor, Michigan, USA), and retatrutide (HY-P3506, MedChemExpress) were dissolved in saline (Otsuka Pharma, Tokushima, Japan)