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Application note about determining Kd for the glucagon GLP-1 receptor with Tag-lite HTRF technology.
References: The Global Use of Medicines Outlook Through 2029
Carolyn Dean MD ND.
When cultured human L-cells were deprived overnight of glucose and serum and then exposed to high glucose, 10% FBS, and -carrageenan (1g/ml) for 10minutes, 1h, and 24h, mRNA expression of proglucagon and secretion of GLP-1 were significantly reduced, compared to control cells not exposed to carrageenan
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