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doi: 10.2337/dc08-1309

Samples then were eluted with elution buffer [1% (w/v) SDS, 250 mM NaCl, 1 mM EDTA, 10 mM TRIS pH 8] for 10 minutes at 65C, and analyzed using SDS-PAGE on an 8% or 12% acrylamide gel
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Additionally, these patients showed higher rates of testosterone deficiency (3.83%) compared to the control group (1.7%)

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