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cAMP accumulation assay FlpInCHO-hGLP-1R and FlpInCHO-pGLP-1R cells were diluted to the density of 6 10 5 cells/ml and INS-1(832/13) cells to 2 10 6 cells/ml in stimulation buffer [phenol-free DMEM containing 5 mM HEPES, 0.5 mM 3-isobutyl-1-methylxanthine (IBMX) and 0.1% (w/v) BSA], and cAMP detection was carried out as described previously 48

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We evaluate the emerging potential of glucagon-like peptide-1 receptor agonists (GLP-1 RAs) as a multifunctional therapeutic strategy to target this metabolic hepatic nexus

Thomas and colleagues at Eli Lilly and Company, [76] investigate the impact of tirzepatide on beta-cell function and insulin sensitivity in individuals with type 2 diabetes

bioRxiv An shRNA screen in primary human beta cells identifies the serotonin 1F receptor as a negative regulator of survival during transplant

[DOI] [PMC free article] [PubMed] [Google Scholar] 108.Reilly-Harrington N.A., Himes S.M., Lauretti-Robbins J., Sogg S