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Expression of target genes was assayed by quantitative real-time/reverse transcription (qRT)-PCR using Bio-Rad iTaq SYBR Green Supermix

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Furthermore, we expanded the search for specific additions to the terms back pain, chest pain, cough, syncope, and urinary tract infection by also including urinary tract infections with specific bacterial etiology, noncardiac chest pain, productive cough, and unconsciousness in addition to the narrower search terms used by Elashoff et al