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Darsalia et al

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and (4) carrying out gradient elution by taking the reverse-phase silica gel filler as the stationary phase, an aqueous solution of ammonium bicarbonate as the mobile phase A and acetonitrile as the mobile phase B, collecting fractions, carrying out rotary evaporation, and carrying out freeze-drying so as to obtain saltless refined peptide of semaglutide, wherein the saltless refined peptide has purity of greater than 99.5% and an individual impurity content of less than 0.1%

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