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Western blotting To analyze the protein expression of GLP-1, GLP-1R, PI3K and p-Akt on different days after NTG intermittent injection, TNC samples were collected 24 h after the first (NTG 1d), the second (NTG 3d), the third (NTG 5d) and the last (NTG 9d) NTG injections
Slides were washed and nuclei were stained using DAPI (Thermo Fisher Scientific 62248, 1:2,000) for 5 minutes at room temperature, washed, and mounted (Abcam, ab104135)

Chronic low-grade inflammation also has a major role in vascular aging, potentially leading to various age-related diseases, including cardiovascular diseases

First, they improve myocardial glucose metabolism by enhancing insulin sensitivity and glucose uptake while reducing lipotoxicity through decreased fatty acid oxidation [74]

Data curation: Fahad S
