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Cells were seeded in 96-well plates at a density of 35,000 cells per well and transiently transfected using Lipofectamine 2000 according to the manufacturers protocol
This suggests that GLP-1R signaling in iTregs may mediate GLP-1R agonists anti-inflammatory effects [45]
Krishnan A, Venkataraman J (2011) Prevalence of nonalcoholic fatty liver disease and its biochemical predictors in patients with type-2 diabetic mellitus
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