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In the cAMP Hunter Semaglutide Bioassay Kit, CHO-K1 cells overexpressing human GLP-1R utilize the receptor's natural Gs coupling to monitor activation

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First, for each sample, mRNA purification, based on oligo dT-based mRNA isolation, was conducted with a starting material of 1g of total RNA using the Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) and following the manufacturers instructions

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