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Inject: Insert the needle at a 90-degree angle into the pinched skin fold

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By using chronic nitroglycerin injection-stimulated mouse model, it was observed that Liraglutide suppressed the nitroglycerin-induced upregulation of microglia in these mice, shortening both total and mean lengths of microglial processes and reduced nitroglycerin-induced upregulation of Iba-1, as well as, IL-1 and TNF- protein levels (Jing et al., 2021)

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