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The pain began abruptly the previous evening, initially attributed to gas, and was unrelieved by simethicone or antacids

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Mitochondria and mood: mitochondrial dysfunction as a key player in the manifestation of depression

They stimulate insulin secretion in response to meals, suppress glucagon release, slow gastric emptying, and reduce appetite through central nervous system pathways

The WT or mutant constructs were transiently transfected using Lipofectamine 2000 transfection reagent as previous described 25