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ICP-MS measurements To quantify the possible presence and concentration of metal ions in GLT25D1/COLGALT1 recombinant preparations, 900 L of a sample containing 4 mg mL 1 GLT25D1/COLGALT1 (purified without usage of divalent ions) were treated with 300 L 65% ultrapure HNO 3 and 200 L 30% w/w H 2 O 2 , made up to 5 mL with ultrapure water and analyzed on a single quadrupole inductively coupled plasma mass spectrometer (SQ-ICP-MS, iCAP RQ Thermo Fisher Scientific), equipped with a quartz cyclonic chamber cooled at 3 C, a MicroMist nebulizer 179 (400 L min 1 ), a quartz torch, a Ni sampler, skimmer cones, and a QCell pressurized with helium (3 V, KED mode)

Glutathione levels can also be boosted by eating certain foods that are rich in sulfur

James SJ, Melnyk S, Jernigan S, Cleves MA, Halsted CH, Wong DH, Cutler P, Bock K et al (2006) Metabolic endophenotype and related genotypes are associated with oxidative stress in children with autism

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5.1.7.4 Oxidative stress and inflammatory pathways The interplay between oxidative stress and inflammation is a defining characteristic of CF lung disease, creating a vicious cycle of damage