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For scanning EM, larvae were then washed, dehydrated stepwise in ethanol of 25%, 50%, 70%, 95%, 100%, 100%, critical point dried (CPD 030, Bal- Tec), coated with gold-palladium (Denton Vacuum) and imaged on a field emission scanning electron microscope (Mira3 FE-SEM, Tescan)
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