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Conversely, the amlodipine-treated group showed normalization of both contractility and relaxation parameters (Figure 5A)

For experiments involving caspase-3/7 staining, CellEvent Caspase-3/7 Green Detection Reagent (Thermo Scientific C10723) and CPP-CAND were added to live cells 24 h before imaging using an Eclipse Ti-U inverted microscope (NIKON)

Upon fluctuating temperatures, ROS also accumulate at the plasma membrane/apoplast interface by NADPH oxidases, superoxide dismutases and peroxidases, and can be translocated to the cytosolic compartment by aquaporins, were they likely take part to stress signaling pathways (Mittler et al., 2012) (Rodrigues et al., 2017)

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The IL-33 model was specifically selected to isolate ILC2-intrinsic responses independent of upstream epithelial-derived alarmin complexity present in the previously employed Alternaria model ( Figure 1 )